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Interstitial area and fiber area were quantified using ImagePro Plus software (Media Cybernetics).
The number of colonies/plate and colony size, expressed as average area, were quantified using RT-Image software [28].
The stained sections were scanned (2.400 dpi), the digitized images of each brain section and the infarct area were quantified using a computerized image analysis program (Image J, USA).
Lesion volume, spared tissue volume, and glial scar area were quantified using GFAP staining and the Cavalieri probe at 4× in all three groups using parasagittal sections, vehicle (n = 5), hFbs (n = 5), and hCNS-SCns (n = 6).
The adipocyte diameter and area were quantified using Image-Pro Plusoftwarere.
Percent tumor burden and all subsequent staining indices per tumor area were quantified using whole slide imaging.
Similar(52)
The lesion area was quantified using ImageJ software55.
Hypoxic area was quantified using HALO™ image analysis platform (Indica Labs).
Whole aorta surface area was quantified using ImageJ software.
For each section, at least five cardiomyocytes sectioned transversely were randomly chosen, and their area was quantified using ImageJ software.
The gene expression area was quantified using ImageJ software (NIH).
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