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Aortic arch lesion area was quantitated using a method similar to that previously described [26], [27].
The image was captured with the use of a Leica MZIZ microscope and digitized, and the oil red O stained lesion area was quantitated using the Image J image analysis system.
Area of positive immunoreactivity, as a proportion of total area was quantitated using ImageJ.
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The fluorescence intensity of OX62 and MHC-II or CD86 coexpression areas was quantitated using ImageJ software (National Institutes of Health, Bethesda, MD, USA).
The area resorbed was quantitated using an Olympus microscope connected to a computer and the OsteoMeasure program (version 3.21; OsteoMetrics, Atlanta, GA, USA).
FRET was quantitated using an Olympus IX83 inverted fluorescence microscope.
Serum OPG was quantitated using enzyme-linked immunosorbent assay.
Radioactivity was quantitated using a γ-counter.
Cell proliferation was quantitated using MTT assay as described [20].
Densitometry was quantitated using Image J software (NIH).
RNA was quantitated using a Qubit™ Fluorometer (Invitrogen).
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