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(5) Prepare surgical area observing sterile conditions meticulously: Use sterile gloves throughout.
Biological methods include introducing pest strains that carry lethal genes, flooding an area with sterile males (as was successfully done for the control of the screwworm fly), or developing new kinds of insecticide based on modifications of insects' growth hormones.
Moreover the extraction took place in a controlled area, in sterile mode, by using disposable and filtered equipments.
In the operating room, approximately 40 mL of packed adipose tissue was obtained by liposuction of the subcutaneous layer of the lower abdominal area using sterile techniques [ 11].
Thus, vaccination started in April 2011 and, according to the manufacturer's instructions, each animal was given 2 doses 3 weeks apart of 4 ml of inactivated phase I vaccine (Coxevac, CEVA Santé Animale, Libourne, France) subcutaneously in the neck area using sterile single-use needles and syringes.
Rinse or scrub the area with sterile water or alcohol to remove any debris.
A 20-page report issued on Monday by the Food and Drug Administration described drug solutions contaminated with germs, rusted and unsanitary equipment, and insects and a bird flying around in areas where sterile products were packaged and stored.
Then for wet areas dry sterile cotton swabs were used.
After 10 minutes, we cleaned the areas with sterile saline solution.
As illustrated in Figure 5D, there are inhibition zones of R. solani in areas containing sterile discs of filter paper on which various concentrations of purified PgD5 were applied.
To protect from infection and further risk of cold damage, gently wrap the rewarmed frostbitten areas in sterile cotton and gauze.
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