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AFM images, in the area of scanning, show no specific surface features such as steps or kinks.
The results are shown with colored solid circles 6 m in diameter (the area of scanning at a depth of 30 m, the average depth in this area).
The coverage area of scanning included the whole cervical spine, from the base of the skull down to the upper dorsal spine, scanned in the craniocaudal direction.
This imaging condition was experimentally optimized based on considerations of time resolution, tracking duration and quantity of mitochondrial (related to area of scanning) as well as photo-bleaching.
For example, if the radiation angle is 12°, the area of scanning is a circle 6 m in diameter at a depth of 30 m, but it is 21 m in diameter at 100 m.
The mesh resolution is adapted to the local needs, higher than background for the area of scanning and highest near the sources and detectors (maximum edge length 0.5 mm).
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Another area of scan's valuable clinical application was in the early diagnosis of testicular abscess in patients with epididymo-orchitis not responding immediately to antibiotic therapy.
The positioning was possible because the area of scan where the photocurrent was first measured could be clearly identified as an indentation made by the tip near the edge.
The radius of the scanning window represents the area of the scanned space, which ranges from 0 to the designated upper limit of the scanning window, while the height of the cylinder represents the scanning interval.
This gives the benefit of scanning an area around that point in one pattern only.
Apart from this, there was no TTP signal on the non-spotted area of the scanned control tissue.
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