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The numbers of particles adsorbed per unit area of leaf differed significantly depending on the location at which the samples were collected.
The plant with higher LMA usually had greater surface area of leaf chloroplast (Smes) or mesophyll cells facing intercellular air spaces per area (Sc), and consequently, mesophyll conductance and photosynthetic capacity (Terashima et al. 2001; Oguchi et al. 2003; Piel et al. 2002).
Prior to drying the surface area of leaf blades was measured for each plant with a Licor LI-3100C (Licor, Nebraska, USA).
The area of leaf 8 from each plant was measured, and the cross-sectional area of palisade mesoplyll cells in leaf 8 was measured with the aid of a microscope.
Leaves from individual trees were harvested separately, and photographed to determine the area of leaf damage as described in [ 77].
A high SLA implies a thinner leaf and a lower density of nitrogen and chlorophyll per area of leaf.
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The leaf area (cm2 of leaf area shoot-1) and leaf area index (m2 of leaves per m2 of substrate) were calculated according to Giraud [ 30].
Among lima bean plants in the field, herbivore damage measured as missing leaf area (of defined leaf stages) showed high quantitative variability (0 741 mm2 corresponding to 0 33% leaf area removed; Fig. 2).
After feeding for 24 h, areas of leaf discs consumed were measured.
The areas stained and areas of entire leaves, both in pixels, were used to calculate percentage areas of leaf stained [12] [13].
Images were then imported in ImageJ (FIJI) (Schindelin et al., 2012) and the total and green leaf area of each leaf were quantified using the built-in threshold and color threshold function, respectively.
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