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The magnetic lasso tool was used to select the area of degradation and average fluorescence intensity within the degraded region.
Of particular attention are the sensitivities and uncertainties related to buffer width in determining area of degradation and the biomass estimates.
The most significant observation at the national scale is that overall area of degradation increased nearly by 50%% in the time period and when associated with biomass estimates, resulted in a quarter of total forest related emissions (Table 4).
However, the area of degradation and intensity of degradation are more similar to the podosomes found in the IC-21 macrophage cells.
Fig. 2A and B clearly show the difference in matrix degradation levels, in particular the area of degradation between trophoblasts grown on 0.2% (Fig. 2A) and 1% (Fig. 2B) over the same incubation period.
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We did see central areas of degradation colocalizing with cortactin, but we also saw degradation at focal adhesions.
Areas of degradation were identified as black holes on fluorescent gelatin and quantified using ImageJ software downloaded from the NIH website.
To quantify the areas of degradation, pictures of fluorescent matrix were analyzed by using ImagePro software, and degradation areas associated with positive cells were calculated in pixels, for a minimum of 25 cells per coverslip.
As shown in Figure 5D, some cells are able to focally degrade gelatin on either citrullinated or untreated fibronectin, although they tend to move over the course of the experiment and do not align perfectly with their areas of degradation.
Using this software, degradation area normalized to the number and area of cells (degradation area per cell) was measured.
Additionally, some perspectives on the challenges and future developments in the area of photocatalytic degradation of antibiotics are provided.
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