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The result indicates a high correlation between the concentration of salbutamol and area of absorbance as measured by HPLC.
LA1C and SA1C are expressed as a percent of total HbA0, based on peak area of absorbance at 415 nm.
The lineal equation was y=9803.3 x−3.2442 and the correlation coefficient was R=1, where y is the concentration of R-salbutamol sulfate in solution (μg/mL) and x is the peak area of absorbance.
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In this study, the areas of absorbance peaks of functional groups are shown in Tables 2, 3, 4, 5.
The obtained calibration curves of the ratio of chromatographic peak areas of absorbance at 357 and at 280 nm vs. nitration degree are nearly the same for BSA and OVA (relative deviations <5%).
Calibration curves plotted as peak areas of UV absorbance at 332 nm against spiked nC60 concentrations showed good linearity over a range of 20 200 μg L−1 after 10-fold concentration by LLE, but only over the range of 0.8 2 μg L−1 for reclaimed wastewater and 0.8 4 μg L−1 for secondary effluent after 1000-fold concentration by SPE.
The size of the nanoparticles can be monitored indirectly by the integrated area of the suspension absorbance spectra (AISAS) in the region where no AuNP absorbance was observed.
For specific activity calculations, the radioactivity of the injected product for the radiochemical analysis was measured with a Capintec R15C dose calibrator (Capintec, Inc., Ramsey, NJ, USA), while the mass of SIG343 and SIG353 was determined by comparing the area of the UV absorbance peak corresponding to the carrier product with a calibrated standard curve relating its mass to UV absorbance.
It is the area under peak of absorbance-time curve was recorded after integration and the concentration was calculated as ppm to obtain a standard curve (Electronic Supplementary Material Fig. S2).
As illustrated in Fig. 4a, the calibration curve of absorbance peak area ratio A357/A280 vs. ND remained essentially unchanged when the chromatographic column of the HPLC system was exchanged, which demonstrates high robustness of our method.
The cross-sectional area of muscle fibres and absorbance values of the final reaction products in muscle fibre sections were determined as follows: Slow, high oxidative muscle fibres were selected close to the skin and horizontal septum.
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