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Integrate peak area for quantitation.
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The accurate mass extracted ion chromatograms were integrated for each identified lipid precursor and peak areas obtained for quantitation.
One can select whether peak intensities or peak areas will be used for quantitation.
Use of the internal standard is important for quantitation as the area sampled during each liquid tip contact depends on the area wetted during that sampling event, the transfer of analyte to the probe, and residue on the probe tip.
For quantitation of percent area occupied, the threshold approach was utilized.
Areas of highest cellularity were used for quantitation.
However, it also shows that there are some key areas, such as inconsistencies in the procedures used for quantitation, that investigators may want to probe further to assure that the highest quality and most useful studies are performed.
The resulting extracted ion chromatograms are used to generate peak areas for transitions of interest and are used for quantitation.
Areas of highest cellularity and expression were used for quantitation, as previously described (Wharton et al, 1998).
For quantitation of TAG1+ commissural axons, relative immunoreactive areas were measured by deriving the ratio of the areas spanned by TAG1+ axons on the electroporated side or contralateral side to the total area of hindbrain as described previously [33].
Areas of different activity as defined above were used for quantitation, from acute, chronic active, and inactive lesions and from control brains (see Supplemental Table S1 at http://ajp.amjpathol.org ).org
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