Then, chondrocytes morphology were analysed by measuring main parameters of the cell shape, including: the area, Feret's diameter, perimeter, and circularity index.
Specifically, we have validated objective parameters associated with morphology, including area, perimeter, Feret's diameter (caliper), circularity, aspect ratio, solidity, and the number of grid crossings between cell-positive area and a grid.
We validated several parameters, including area, perimeter, Feret's diameter (caliper), circularity, aspect ratio, and solidity, providing quantitative information on M/M morphology over wide tissue portions.
Once segmented, the objects meeting the minimum size to be analyzed were measured for the following parameters: area, perimeter, Feret's diameter (caliper), circularity, aspect ratio, and solidity.
The 3D scatter plot locates an elongated structure (e.g. interconnected islets) at a point with a greater value in area and Feret's diameter and a smaller value in circularity compared to spherical shaped islets and small clusters.
Fiber area and Feret's diameter of cross-sectional muscle fibers and the mean gray value of NADH transferase activity staining images were analyzed using ImageJ software (National Institutes of Health).
Shape descriptors (area, perimeter, circularity, Feret's diameter, aspect ratio, and solidity) were quantified on 179 × 133 μm fields (A).
We did not detect significant differences in area, perimeter, and Feret's diameter between pMCAo or TBI and naïve cortex.
These technical limits could lead to underestimation of area, perimeter, and Feret's diameter in steady-state microglia of naive animals, resulting in a lack of sensitivity.
Leaf area, perimeter and Feret's diameter (i.e., parallel lines touching opposites ends of the leaf) were calculated from the scanned leaves using ImageJ software [39].
Mouse quadriceps were stained for hematoxylin and eosin and the average cross-sectional area (A ) and Feret's diameter (B ) of muscle fibers were quantified at ages indicated.
