Then, 500 μl of dichloromethane (CH2Cl2) (Sigma) was added and mixed by vortexing, followed by centrifugation for 5 min. After centrifugation, the organic (VP, lower phase) and aqueous (liposome, upper phase) layers were visibly separated.
Testis, ovary, muscle/test (calcareous skeleton), gut, lantern (jaw muscles and ligaments) and coelomocytes (immunocytes in the body cavity fluid) were the six tissues that can be visibly separated from each other for RNA extraction.
The results showed that all stress-treated datasets tended to be grouped together and can be visibly separated from the controls, suggesting the stresses of iron depletion and copper toxicity have caused significant changes at the transcriptional level (Additional file 1: Figure S1).
A total of 125 of the subset of 288 SNP loci for which we developed primers (43%) produced clean amplification signal, yielding genotypic clusters that were visibly separated from each other and could be scored following PCR amplification and SNP genotyping.
Continue to pack lightly until the wings are visibly separate from the body.
Here, we investigate the effect of chromosome damage imposed after anaphase has begun, as defined by the morphological criterion that the chromosomes are visibly beginning to separate.
A few are visibly shaking.
Still, investigations and convictions are visibly increasing.
Walkways and railings are visibly sagging.
Whenever hands are visibly dirty or visibly soiled a hand wash should be performed [ 1].
