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This results in an increase in the rate at which the affected DNA molecules are transcribed to produce messenger ribonucleic acid (mRNA) molecules and an increase in the rate of synthesis of the protein (translation) coded for by the DNA (by way of the mRNA).
In contrast, genetic information refers to the linear sequence of codons along the DNA, which (in the simplest case) are transcribed to messenger RNA, which are translated to linearly order the amino acids in a protein.
There are several approaches that can be followed, such as Southern/Northern blotting, fluorescent in-situ hybridization and quantitative real-time PCR (qPCR), optionally including a reverse transcription (RT) step (RT-qPCR) to quantify RNA species such as messenger (mRNA) or ribosomal RNA (rRNA) which are transcribed to and measured as complementary DNA (cDNA).
The Guarente team's work also suggests that whatever the protein encoded by the normal form of the Werner's gene does to stave off premature aging, it does it in the nucleolus, a specialized part of the nucleus where ribosomal genes are transcribed to make the protein-synthesizing machines known as ribosomes.
SREBPs are generated from two genes, SREBF1 and SREBF2, that are transcribed to form a number of different mRNA and protein species [4] [8].
However, given the incomplete nature of the paternal imprinting during the 4 cell to blastocyst stage it is most likely that sufficient levels of wild type Bcor are transcribed to support development.
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One such tool is the microarray, or expression chip, which can display which of a cell's genes are switched on and being transcribed to make protein.
Afterwards, the interviews were transcribed to analyze the collected material.
The focus group interview was transcribed to analyse the findings (Eisenhardt 1989; Yin 2009).
The interview with Aidan lasted approximately 25 minutes and was transcribed to 13 pages of text.
The amplified region was cloned and the plasmid was transcribed to generate calibration standards for quantification.
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