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In order to further quantitatively evaluate the concentration of Verapamil, the oxidized Verapamil products are then detected using an electrochemical microchip with a gold nanoelectrode ensemble electrode (GNEE) as the working electrode.
Hyperspectral images are produced by imaging spectrometers, which use an optical dispersing element (e.g. grating or prism) that splits the light into many wavelength bands, which are then detected using 100 1000 s of detectors (e.g. across a CCD chip).
Following a stringent wash to remove non-covalent substrate-modifier interactions, the PTM-conjugated substrates are then detected using 'binders' (e.g. antibodies or streptavidin) labeled with fluorescent dyes and the protein microarrays analyzed using a fluorescence slide scanner.
The bound antibodies are then detected using a secondary antibody to IgG (bound with Cy3).
For the indirect GIFT the patient's serum is placed in contact with control neutrophils, and autoantibodies are then detected using a fluorescinated human anti-immunoglobulin antiserum.
The principle of this novel ELISA is that serum proteins containing nitrotyrosine residues are captured on the plate using an anti-nitrotyrosine antibody and the subset of captured proteins that contain histones are then detected using a polyclonal anti-histone antibody.
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Oligonucleotide functionalized magnetic microbeads are used to capture the target DNA which is then detected using biotinylated detection probes and a streptavidin-coupled label.
The signal was then detected using Chemiluminescent Detection System (PIERCE, Rockford, IL).
Pretreatment of slides with Cell Conditioner 1 (EDTA) MILD using the Ventana BenchMark XT stainer system was followed by incubation with the primary antibody for 32 minutes at 37°C, which was then detected using Ventana UltraView detection with Ventana DAB.
The signals were then detected using Western blotting detection reagent.
The proteins were then detected using the ECL detection kit.
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