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These genes would clearly not be suitable to normalize data obtained from these particular experimental conditions, even if their overall expression stability is high.
Cherries are suitable to candy.
Of the eight housekeeping genes studied, peptidylpropyl isomerase A (PPIA also called cyclophilin A) and ribosomal protein S13 (RPS13), especially in combination, were best suitable to normalize gene expression in cancer and normal tissue [ 23].
The candidate reference gene DBP (DNA binding protein) was considered the most suitable to normalize expression data in samples of strawberry cultivars and under drought stress condition, and the candidate reference gene HISTH4 (histone H4) was the most stable under osmotic stresses and salt stress.
In summary, these results clearly show that the information provided by spikes' signal is reproducible, characterized by low variability (that can be further reduced by filtering our outliers values) and suitable to normalize the results of our test-array.
The highly significant correlation when the real time PCRs were normalised to either β-actin or ELF-1α shows that both these control genes are suitable for normalizing the IL-1β and IFN-γ responsive genes which are changed in expression.
In summary, our data indicate not only that TFRC and RPLP0 are suitable reference genes to normalize gene expression level in qRT-PCR experiments in hypoxic and/or hyperglycemic HUVEC cultures but also that commonly known reference genes always need to be validated for the given experimental conditions.
Therefore, no single reference gene with stable expression is suitable for normalizing all expression data obtained from various experimental conditions.
We demonstrated that the spike-in system was suitable for normalizing our platform and determining the threshold for discriminating the hypoxia modulated genes.
The combination of HMBS, B2M, SDHA and GAPDH was suitable for normalizing gene expression data among five liver cancer cell lines, yielding a V4/5 value of 0.175, which is close to the cutoff value 0.15.
HMBS, GAPDH and UBC were identified to be suitable for the normalization of gene expression data among tumor tissues; whereas the combination of HMBS, B2M, SDHA and GAPDH was suitable for normalizing gene expression data among five liver cancer cell lines, namely Hep3B, HepG2, HuH7, SK-HEP-1 and SNU-182.
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