Then, affine registration to MNI space was applied, and the non-linear deformation parameter was subsequently calculated using the high-dimensional Dartel algorithm81.
The CAE activities were subsequently calculated using the standard curve.
The affinity of cells to each solvent was subsequently calculated using the following equation: Affinity % = [1 − (A1/A0)] × 100.
The FAE activities were subsequently calculated using standard ferulic acid and methyl ferulate curves according to the equation previously published (Yue et al. 2009).
The tree was subsequently calculated using the Neighbor program and visualized using the Njplot program [21].
Relative or fold change in expression was subsequently calculated using the formula 2−ΔCt or 2−ΔΔCt, as described in [69].
The complete dataset, consisting of 144 CEL files, was downloaded and expression scores were subsequently calculated using the robust multichip average (RMA) method.
Values for each endocannabinoid and NAE analogue were subsequently calculated using ratios to the deuterated internal standards to calculate absolute concentrations, expressed as picomoles (pmol) of EC metabolite per ml of plasma similar to current methods [30].
Left ventricular mass was subsequently calculated using Devereux's method.
Relative expression was subsequently calculated using the 2-ΔCp approach.
