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Sections were taken every 7 µm, but only sections from 150 µm to 550 µm were subsequently affixed to glass slides for staining as these contained the articular cartilage.
For protein binding microarrays, double-stranded DNA of known sequence is affixed to the microarray surface and the adherence of a fluorescently labeled protein preparation of a TF (or frequently just the DNA-binding domain from a TF) is measured; the bound sequences are subsequently analyzed to determine the DNA sequence patterns targeted by the protein.
PCL nanofiber coated TCPS was then placed in a vacuum overnight to insure removal of residual solvent [ 44], and then plasma treated (Harrick Plasma) to promote cellular attachment [ 36], and subsequently affixed to 24-well cell culture plates for cellular experiments.
Images were subsequently opened in the Metamorph software and a grid was affixed on the image defining three regions of interest (ROIs).
He was subsequently fired.
He was subsequently arrested.
Monroe was subsequently arrested.
It was subsequently closed.
Goodman was subsequently jailed.
He was subsequently cleared.
Both were subsequently bailed.
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