Over the next 3 years, >30,000 clinical specimens from US patients with suspected tickborne diseases will be screened by using high-throughput molecular methods designed to detect bacteria, followed by use of genomic sequencing to characterize detected pathogens.
This chemical library was screened by using a combination of ligand guided and structure guided techniques.
The targets sequences were screened by using of standard in silico tools and immunoinformatic web servers.
These compounds were screened by using Molinspiration software for their physicochemical properties and all the compounds showed good oral bioavailability.
The library was screened by using different molecular simulation protocols, resulting in the selection of 15 peptide candidates.
All variables affecting the extraction process (sodium hydroxide concentration and volume, ultrasound power, cycles and time) were screened by using the analysis of variance and multivariate design.
Methods: Three hundred children (599 ears) were screened by using 3 test modalities, pure-tone audiometry, distortion product otoacoustic emissions (DPOAE), and tympanometry.
Variables affecting the extraction procedure were screened by using a 25−1 fractional factorial design and among them; sample amount, solvent volume, solvent ratio and extraction time were optimized by applying a rotatable central composite design (CCD).
All variables affecting the extraction process (sodium hydroxide concentration and volume, ramp and hold times, temperature and number of repeated extractions) have been screened by using a Plackett Burman design (PBD) as multivariate approach.
Homozygous transgenic rice plants were screened by using 70 mg/L hygromycin B (InvivoGen, USA).
