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This is because slightly larger amounts are required for subsequent analyses such as specialized biochemical measurements (ferritin, cortisol) and genetics (C282Y and H63D of the HFE gene).
Taken together, these results indicate that the increased production of intracellular ROS and the activation of p38 MAPK pathways are initial signalling events in the regulation of scavenger receptor expression by glucose that are required for subsequent activation of NF-κB.
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If Ubp2 is not required for plasma membrane ubiquitination and internalization, it must therefore be required for subsequent steps of trafficking.
Initial BACE1 processing of APP is required for subsequent γ-secretase mediated production of the Aβ40 and Aβ42 peptides that are the major components of amyloid plaques.
Together these results suggest that phosphorylation of GCP-WD at serine 418 is required for subsequent phosphorylation at other sites, including threonine 557.
These results suggest that RACK1 may both interfere with agonist-induced sequestration and be required for subsequent targeting of internalized M2 receptors to the degradative pathway.
Rab5 mediates fusion of early endosomes with the sorting endosome, an event required for trafficking through any endosomal pathway, and Rab11 is required for subsequent routing of an endosome through the recycling pathway [14].
Adhesion process of bacteria occurs at the early stage of infection and is essential for its colonization, and in turn, colonization may be required for subsequent development of symptoms of diseases.
Interestingly, this type of muscle and tendon interaction also operates in Drosophila, where tendon precursor cells are specified in the absence of muscles, but muscle attachment is required for subsequent differentiation of Drosophila tendon cells [74], [75].
The association of APBB2 with APP is required for subsequent processing of APP CTF's by gamma secretases, and overexpression of APBB2 leads to the increased generation of the amyloidogenic Abeta42 fragment [27].
Although several viral proteins are capable of mediating attachment, binding of the gD glycoprotein to one of its receptors is required for subsequent membrane fusion and productive HSV infection.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com