AR-A014418 was reconstituted in a modified solution (Noble et al., 2005) that contains 40% poly ethylene glycol) 400 and PBS, and dimethylamine was omitted from the original recipe because of its high toxicity in vivo.
The humble hooded anorak – the surprise standout trend from the month of shows – was reconstituted in a mashup of technical and luxurious fabrics.
In any democracy, this would be hugely problematic so the bar for such a decision should be set exceptionally high.The fallout at home would be serious, even if the AKP were reconstituted in a different form.
Protein extracts were reconstituted in a sample buffer [0.062 M Tris HCl, 2% sodium dodecyl sulfate (SDS), 10% glycerol, and 5% β-mercaptoethanol].
Core components in ABA signaling, including OsPYLs, OsPP2C, OsSAPK2, and OsOREB1, have been identified in rice, and the ABA signaling transduction pathway has also been reconstituted in a protoplast system (Kim et al. 2012).
This was repeated at least one more time, after which the sample was reconstituted in a known volume of 0.15 moles per liter (M) nitric acid (trace metal grade).
We embarked on an examination to see whether a complete functional bacterial luciferase pathway can be reconstituted in a transplastomic plant to produce both the luciferase and luciferins.
For these experiments sPB1-F2sf or sPB1-F2pr8 wereconstitutedted in a planar lipid bilayer with 500 mM KCl on the cis and 500 mM CaCl2 on the trans side.
Before reading, the beads were washed once with PBS/0.05% Tween 20 and were reconstituted in a final volume of 70 µl HPE/S.
The beads were reconstituted in a 20-µL solution containing 0.05 M Hepes (pH 7.3), 25 mM dithiothreitol, 20 µM ZnCl2, 1 mg/mL bovine serum albumin, and 50 pmol/µL of peptide substrate.
