Sentence examples for are randomly integrated from inspiring English sources

In most cases, multiple copies of the transgene, arrayed in a head-to-tail manner, are randomly integrated in the host genome, which may cause transcriptional interference that represses expression [15] [17].

For example, site-specific insertion of cassettes can be carried out in preliminary randomly integrated recombinogenic sites [ 3], or the "marked" cassettes can be randomly integrated, followed by excision of the marker by a site-specific recombination system [ 1].

Southern blot analysis of transformants indicated that the plasmid vector was randomly integrated in multiple copies into the genome of C. acutatum.

This can be expected because the construct carrying the genes is randomly integrated in the genome of A. niger and its copy number varies.

Another "Super Pgap" mutant library was further constructed where the strong native promoter Pgap of Z. mobilis was randomly integrated throughout the genome of Z. mobilis 33C via an in vitro transposon mutagenesis system.

Building upon these earlier attempts, in the present work we improved transformation conditions through the development of two new vectors and also gained insight into the fate of the transferred T-DNA, which was found to be randomly integrated, with a preference for repeat element-containing genome sites.

Since the Cre cDNA has been randomly integrated into the genomic DNA [11], it is likely that not all copies integrated into the genome have the same transcriptional activity.

These insertion sites suggest that the transgenes were randomly integrated in the chromosomes.

These samples were randomly integrated into several runs including all steps from pre- to postanalytics to include all influencing factors.

The long-term stability of transgenes, which have been randomly integrated into the genome, and the perpetuation of their expression are beneficial for any genetically engineered organism.

To achieve this task, a promoterless gene encoding a chloramphenicol acetyltransferase (cat), was randomly integrated into the S. gordonii chromosome and transformants were selected for chloramphenicol resistance.