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Third, the CNVs discovered by each platform are relative to the animals that are probed against.
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T cells from mice that were immunized with a DNA vaccine encoding MPT-51 were probed against MHC-compatible cell lines retrovirally transduced with overlapping gene fragments encoding 120 140 amino acids of the MPT-51 molecule.
Using an indirect ELISA setup where OMAB was probed against various Aβ species, we showed that OMAB exhibited considerable selectivity towards oligomeric species.
Three shRNA constructs were probed against Ras-GRF1 and Ras-GRF2 genes in primary cultures.
Membranes were probed against RIP1 (BD Transduction Laboratories, #610458), caspase-8 (1Cell (Cell Signaling, 9746) and (BD Transduction Laboratories, #610399).
Fractions were probed against peptide-generated antibodies against the Perforin-2 domain (C93), and peptide generated antibodies against the MACPF domain (C186, C252).
To explore whether the inhibitory effect of MELK-T1 on cell growth is dependent on the genetic background, MELK-T1 was probed against a panel of 81 solid tumour cancer cell lines (© Oncolead GmbH & Co. KG).
Southern blots were probed against CaLCuV DNA A common region (CR) (bottom panels); the viral populations are indicated as the replicative intermediate open circle (OC), linear (L), super coiled (SC) and the infective particle ss DNA.
Array-based assays use reverse hybridization in which a labeled sample is probed against a series of proteins or nucleic acids that are bound to a solid support, such as a nylon membrane or silica.
Yeast micro-arrays were probed against total RNA extracted from three independent cultures of strains BMA64-URA3 (wild type) and FL-M9 (rpb1-L1397S) grown on SD+aa at 30 °C, to an OD600 of 0.55.
Blots were probed with antibodies against p62, GFP, and β-actin.
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CEO of Professional Science Editing for Scientists @ prosciediting.com