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In combination with previous studies about the complete delignification resulting in the lower sugar release [ 30], this finding could have significant implications, for example, on how genetically modified low-lignin plants are pretreated for enzymatic hydrolysis.
For controls, terminal deoxynucleotidyl transferase enzyme is either omitted from the labeling solution (negative control), or sections are pretreated for 30 min with DNAse I (Roche; 3 U/ml) in 50 mM Tris-HCl, pH 7.5, 1 mg/ml BSA to induce DNA-strand breaks (positive control).
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This work was conducted to evaluate an enzymatic hydrolysis of kenaf stem (that has been pretreated) for xylooligosaccharides (XOS) production.
In the DRIFTS cell, powder adsorbents were pretreated for 1 h at 200 °C under a nitrogen flow of 30 mL/min.
To test the effects of BNP on the BKCa currents, BNP (100 ng/ml) were pretreated for 3 h in culture medium.
Cells were pretreated for 1 h with 5 mM of NAC before 6 h co-exposure with 12 μg/ml ZnO NPs (right).
Astrocytes were pretreated for 1 h with 0.05, 0.5, and 5 mM of NAC before 6 h co-exposure with ZnO NPs (12 μg/ml).
The astrocytes were pretreated for 1 h with 5 mM of NAC before 6 h of co-exposure with ZnO NPs (12 μg/ml).
The waste cooking oil was pretreated for purification firstly, and then reacted with monoethanolamine, or diethanolamine, or triethanolamine at a certain temperature and turns into the alkanolamides.
Astrocytes were pretreated for 1 h with 5 mM of NAC before 6 h of co-exposure with ZnO NPs (12 μg/ml).
Astrocytes were pretreated for 66 h with 5 μg/ml IIIM1 (4.95 μM) followed by 6 h exposure to MeHg (5 μM).
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