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Thus, 21-nt sRNAs with 5'-terminal uridine (5'U) are predominantly bound to AGO1 and, in one specific case, to AGO7, with 5'A to AGO2, and with 5'C to AGO5, whereas 24-nt sRNAs with a 5'A are bound to AGO4.
However, two factors question if these ionic conditions correctly reproduce the environment of nuclei in vivo: the small-scale motion and conformation of chromatin in vivo are not reproduced in isolated nuclei, and experiments and theory suggest that small ions in the cytoplasm are not free in the soluble phase but are predominantly bound to macromolecules.
This indicates that in cells where the PARP activity was not inhibited, Ctcf, Parp1 and Dnmt1 are predominantly bound to DMR1 DNA fragments containing the unmethylated HpaII sites.
Noteworthy, the proteins of each of these pairs have also similar diffusion speeds, as measured by FCS, suggesting that they are predominantly bound to each other, rather than with other proteins, in the cytosolic pool.
Cardiac troponins I and T are predominantly bound to the contractile apparatus in cardiomyocytes, but a minor fraction of these molecules are found freely soluble in the cytosol [ 10].
Vitamin D metabolites in the circulation are predominantly bound to DBP; however, a proportion of an oral dose, particularly for the less-polar vitamin D, will initially be associated with chylomicrons that may further influence initial metabolism and sequestration into body tissues.
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Despite these differences the dissolved fraction (fd) and partitioning coefficient (Kd) distributions are similar, illustrating that P is predominantly bound to PM fractions.
An analysis of the eluates revealed that Fe is predominantly bound in insoluble degradation products, whereas a considerable amount of Mn is in solution.
Whereas Sb was mainly bound to the residual fraction and partly also in the reducible fraction (Fe-oxides), As was predominantly bound to Fe-oxides (up to 71% of the total As content).
Co-labeling with HKI-CFP and MitoTracker-YFP (Fig. S1) supports the interpretation that HKI, even when overexpressed, is predominantly bound to mitochondria.
Assuming that the solubilized heme c in photoheterotrophically grown cells is predominantly bound to the tetraheme cytochrome the ratio would be 10 BChl a to 0.3125 reaction center cytochrome c or 32∶1, which is the theoretically expected value for a photosynthetic reaction center embedded in a LH1 complex containing 28 BChl a molecules.
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