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These proteins are phosphorylated when the mTOR pathway is activated.
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- In support of this, Taguchi et al. showed that the mitochondrial fission protein, Drp1, is phosphorylated when the cell enters mitosis, leading to extensive mitochondrial fragmentation.
One explanation for the comparatively subtle effects of the Exo1-4S∷A and Exo1-4S∷E mightions might be that other serine (threonine or tyrosine) residues were phosphorylated when the primary kinase targets had been mutated.
Position 704 is on the cytoplasmic side of PERK, a segment of the protein that is phosphorylated when activated and that has the active site for phosphorylating eIF2α.
Based on the protein microarray analysis, validated in N. benthamiana, AtWRKY53 was phosphorylated when co-expressed with the AtMKK7/AtMPK7 module [ 57].
FAK is an essential component of integrin signaling and is phosphorylated when cells are adhered to the extracellular matrix.
See related research article by Jin et al., http://breast-cancer-research.com/content/12/6/R96 In the previous issue of Breast Cancer Research, Jin and colleagues [ 1] demonstrated that the lipogenic enzyme fatty acid synthase (FASN) is phosphorylated when it is in complex with the tyrosine kinase receptor HER2.
These biochemical assays indicate that while the PHD mutant can be phosphorylated when overexpressed, modification by ubiquitin is abrogated in the PHD mutant.
Intriguingly, the S. aureus IsdB protein, which is phosphorylated in S. aureus, was also found to be phosphorylated when heterologously produced in L. lactis, albeit not on the same Tyr residue.
Of note, OA partially restored the levels of pERK in Mel-RM cells with ERK knockdown by siRNA, suggesting that the minimal residue of ERK was phosphorylated when PP2A was inhibited.
We did not observe any phosphorylation of Ser396/Ser404 by endogenous Drosophila kinases but this epitope was phosphorylated when hGSK3β was co-expressed with Tau.
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