Sentence examples for are needed for total from inspiring English sources

Prior to isolating total RNA from cultured cells, we collect cells from culture flasks by standard methods, pre-homogenize them in 1 or 2 ml of Trizol® by hand-pipetting, then store the resulting cell pellet-Trizol® pre-homogenates at -80°C until they are needed for total RNA isolation.

Compared to the shell catalyst, the conversion of CO started at lower temperatures when using the homogeneous catalyst, but higher temperatures were needed for total conversion.

A major feature of melioidosis is the difficulty in achieving complete bacterial eradication following an episode of infection and an extended period of antimicrobial treatment is needed for total clearance.

The velocity follows these trends: 240 days are needed for the total retention of europium for temperatures over 200 °C; below 150 °C, significantly longer reaction times, on the order of three years, are required to complete the reaction.

As a result, 150 subjects per practice are needed for a total of 7500 subjects from 50 practices.

Add cups, mixing straws, and napkins to your purchase list, calculating how many are needed for the total number of guests expected.

To show this difference at the 5% significance level with 90% power, valid data from at least 543 participants in each arm of the trial are needed for analysis (1086 total).

Now, comprehensive information provided by whole-body and real-time observation of Cd movement in intact plants during vegetative and reproductive stages are needed for understanding the total plant system that leads to the difference of Cd concentrations between various cultivars.

a. Two control and four experimental wells (6 wells total) are needed for each cell line in Cohorts 1 3.

And financial incentives to encourage workers to retire later could also boost the labour supply: only 60% of men and 30% of women aged over 50 have jobs.Moreover, Mr Cai's estimate of China's labour surplus assumes that 180m workers, or 24% of total employment, are needed for farming.

In total, 788 participants are needed for the study.