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While simply increasing template concentrations might be useful when dealing with modern and invasively obtained DNA sources, such a strategy would not work for low copy viral RNA from highly degraded archival FFPE tissues, especially if the same extracts are needed for multiple tests/targets.
In mammals, three isoforms of α subunit (α1, α2, and α3) and two isoforms of β subunit (β1 and β2) that are needed for multiple cellular functions have been characterized.
This approach will be most useful for samples that have limited availability but are needed for multiple analyses, especially for the correlation of proteomics data with histology and immunohistochemistry. Tissue analyses, including histomorphological and immunohistochemical approaches, form the basis for most diagnostic analyses in anatomic pathology.
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We used transversions rather than total substitutions as (a) they occur at a slower rate than transitions, (b) they provide a simpler molecular clock as no assumptions or modeling of transition/transversion rates are needed for multiple-substitution correction, and (c) transversions are insensitive to local variations in GC content and unaffected by methylation effects.
Despite the importance of targeted preschool, which limits participation to children from low-income families or children with other risk factors, universal public preschool is needed for multiple reasons.
Leadership support was (and, in general, is) needed for multiple aspects of the DMP: for the successful implementation of a health ICT system [ 15], to guide the vision of the improvements in chronic care treatment and management [ 2, 16], and to facilitate change in the healthcare delivery [ 17].
New methods are needed for combining multiple densities, for example 3,000, 50,000, and 500,000 markers, in the same dataset.
As childhood cancer survivors remain a vulnerable population, targeted health interventions are needed for this multiple risk-taking group.
Larger mapping populations are needed for detection of multiple loci by interval mapping methods [ 33], and these are the larger mapping populations in our study.
One reason is that multiple specific autoantibodies are needed for diagnosing UCTD since it includes multiple autoimmune diseases.
We conclude that multiple platforms and multiple models are needed for model applications for different purposes.
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