Prepregs were manually prepared and stored in controlled environment prior to use.
18F-AmBF3-losartan was manually prepared in ~35 minutes, with 11 – 18%% radiochemical yield, > 97% radiochemical purity, and 1.8 – 2.9 GBq/μmol specific activity.
While the descriptions of the settings (often accompanied by pictures) could be quite elaborate, much as in adventure fiction, the input options available to the player were, and have largely remained, restricted to simple utterances of the sort that can be anticipated or collected in pre-release testing by the game programmers, and for which responses can be manually prepared.
The reaction mixtures were prepared in triplicate in two 384-well plates, A and B. For patient samples n. 104 to 113, real-time PCR reactions were manually prepared using TaqMan® individual MicroRNA Assays (Applied Biosystems) for miR-127 (P/N 4373147), miR-154 (P/N 4373270), and miR-16 (P/N 4373121).
All peptide-based samples were manually prepared and run in triplicate with a general standard deviation of <0.2 Da per time point.
Silica gel and acidified silica gel columns were manually prepared by filling an amber glass column (i.d. 0.6 cm) with 1.8 g of silica or 5.0 g of acidified silica with a plug of glass wool at the bottom.
The food pellets were manually prepared by baking NR into the powdered food as described previously (Canto et al, 2012), and the protocol followed this published protocol in detail.
The hemimandibles were then manually prepared from the heads and allowed to dry.
All the plots were manually dug and prepared for the sowing of wheat crop.
Following hybridization, slides were manually washed and prepared according to the manufacturer's recommendation before image capturing using the AB1700 reader.
Testosterone implants were manually and aseptically prepared in the laboratory using silicone tubing (0.058 inch ID/0.077 inch OD; Dow Corning) filled with crystalline testosterone T-15000; SigmAldrichch, UK), and sealed with adhesive silicone type A glue [ 45].
