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They are maintain on a BalbC genetic background at the Biological Resource Center, (Biopolis, Singapore) and all animal experiments followed the guidelines set by the National Advisory Committee for Laboratory Animal Research.
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Both lines of mice are maintained on a C57Bl/6 background.
All strains were maintained on nutrient agar.
All bacterial pathogenic strains were maintained on nutrient agar slants.
Pure colonies obtained were maintained on agar slants at 4 °C.
Culture was maintained on potato dextrose agar slants at 4°C.
Lactobacillus sp. was maintained on lactic agar media (pH 6.0).
The strain was maintained on Sabouraud's dextrose agar (SDA).
Bacterial cultures were maintained on LB agar slants.
Patient was maintained on methotrexate 10 mg weekly.
The samples were maintained on ice during transportation until processing.
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