The most frequent difficulties reported (these are not mutually exclusive) by students surveyed in 1998/99 were: "lack of reference materials/literature" (66% of students), and "financial problems" (58%).
Consequently, SLAF-seq is an ideal strategy for large-scale marker developing and genotyping for species which is lack of reference genome information, such as wax gourd.
The microbial community in soils especially in pristine environments is dominated by novel microorganisms, which are lack of closely related reference sequences in known public genomic databases.
The common issues with the web-based tools are lack of flexibility (parameter options, outdated reference genome or miRNA annotations), reliability (server down or not functional at all), and control of sensitive patient data.
The most frequent difficulties reported by the students during the medical training were: "lack of available reference books" (66%) and "financial" (58%).
Today the themes related to urban design are lacking of appropriate legislation references in Italian urbanism, falling in between the strictly urban issues related to the city and those concerning individual building objects.
Although health care providers may have tools to educate their patients about the impact of quitting smoking, they may still be lacking points of reference and achievable benefits of behavioural change that can be discussed with patients.
That is because there is a lack of reference data compiled from other people's DNA.
As Norris & Olson [ 9] have identified, a limitation to using Dartfish Video Software Solutions is that the number of studies tracking movements of participants with varying pathologies published today is small, and hence there is a lack of reference values or parameters for subject variability/error comparisons purposes.
A major limitation in the development of novel HIV-2 diagnostic assays is the lack of reference materials that can be used to evaluate, optimize, and monitor assay performance.
