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This includes a two-step procedure, where all reads are initially aligned to the reference genome with Bowtie.
In RepEnrich, reads are initially aligned to the unmasked genome and divided into uniquely mapping and multi-mapping reads.
Multiple sequence aligners use evolutionary information that is provided by the guide-tree, a cladogram that dictates in which order the sequences are initially aligned to each other.
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All ACSL amino acid sequences retrieved in the database mining were initially aligned in MAFFT alignment software using default parameters [ 35] and manually curated with the exclusion of regions of uncertain homology, gaps, and of partial sequences.
Raw reads were initially aligned to the full genome using an alignment score of 260 or greater, which for this dataset allowed for only one SNV per read.
For phylogenetic analysis, study sequences and relevant reference sequences were initially aligned with CLUSTALw [ 31] and the alignment was trimmed to a uniform length.
GH32 and RP amino acid sequences from fungal genome data, and the GH32 group 1 nucleotide sequences from genome and experimental data, were initially aligned using MUSCLE [ 41], and the resulting alignments were manually edited.
Sequences were initially aligned using MacClade 4.06 with final editing of the alignments by hand in PAUP* 4.0b10 [ 56], and the alignment was deposited in TreeBase [, accession # S2303].
All sequences were initially aligned with CLUSTAL W 1.83 [ 29] and the preliminary alignments were manually improved in SeaView [ 30].
> -wrap-foot> The 13 PCGs and the two rRNAs were initially aligned with MATTF v6 (Katoh and Toh 2008) and then the alignments were manually optimized.
Sequences with no known 3D structure were initially aligned by (i) identifying the most conserved segments in the PSI-BLAST HSP alignments with the Ballast [55] program and (ii) using these conserved segments as anchors for the progressive multiple alignment strategy implemented in DbClustal [56].
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