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The second cluster we identified contains genes which are induced over a longer time frame (Fig. 2, turquoise cluster).
Data from time-course gene expression experiments have the potential to reveal regulatory interactions as they are induced over time.
Mesp1 is transiently expressed ((Saga et al., 1999) and Figure 1 figure supplement 1B), thus all of our clones are induced over a narrow window of time during gastrulation.
If toxic proteins such as BRC4 are induced over a long time, the rate of Dox resistant population appears increased because of the ensuing cell death (Fig. 3B, 48 h time point).
Third, we have counted the number of clones in embryos at E8.5 and at E14.5 and we see a similar distribution, suggesting that no additional clones are induced over this time window.
While we have not used a conditional Cre allele to control the timing of Cre activity, we believe there are several lines of evidence that support the conclusion that Mesp1 Cre is active (and thus clones are induced) over a very narrow temporal window (from ∼E6.0-E7.5 ∼E6.0-E7.5
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By exposing Daphnia to UV radiation, the synthesis of melanin can be induced, over-riding any probable down regulation due to stress.
Controlled haemorrhage (45% of estimated blood volume) was induced over 15 minutes and sustained shock (MAP target 40-45 mmHg) was maintained for an additional 75 minutes.
β-Galactosidase could be induced over 300 fold with this system, and the extent of induction could be varied depending upon the amount of tetracycline added.
For the longest gaps, successful esophageal growth was induced over days in 38 patients by internal (5), external (23), and mixed internal/external traction (10).
ODC induction at the highest levels was accompanied by a slight decline in intracellular spermidine and spermine levels and this observation was supported by the finding that SSAT activity was induced over 40-fold under these conditions.
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