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An example workflow for the identification of cis-regulatory TFBSs linked to a disease is outlined in the following section, and is illustrated in Figure 2. Given a set of high-throughput sequencing data from an individual, the short sequences are individually aligned to a reference human genome sequence.
Although the GroES-binding sites in R-open are individually aligned 100° from their final orientations in the plane of the GroEL-GroES-domed ring (compare Figures 6C and 6D), the mobile loops are sufficiently long and flexible to readily dock into the R-open ring from their unbound state (Hunt et al., 1996; Landry et al., 1996).
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Sequences belonging to subtypes B, C, and 01_AE were individually aligned to HxB2 (using pairwise alignment functions from SeqAn (Döring et al., 2008)) to construct a summary of diversity within subtypes in HxB2 coordinates.
For analyses that included Acorus, the Acorus genes were individually aligned with the individually extracted gene alignments from the Goremykin et al. [ 19] dataset using CLUSTALW [ 88], and the resulting gene alignments were concatenated to regenerate a matrix identical to the original except for the extra row containing Acorus.
These gene families were individually aligned and concatenated together to give an alignment of exactly 38,000 amino acids in length.
The mitochondrially-encoded proteins were individually aligned with MATTF v.6 (Katoh et al., 2005), and each alignment was manually optimized.
Amino acid sequences for each gene were individually aligned using Clustal X under default setting, and then concatenated into single alignments for phylogenetic analyses.
To this end, the products of the comQXP locus were individually aligned with homologues, B. licheniformis strains were usually found in the same cluster, alongside the less well-described Bacillus sp. BT1B_CT2.
UTR sequences were individually aligned using MUSCLE.
The eleven influenza A proteins of each subgroups were individually aligned using MUSCLE 3.6 [66].
The DNA sequences of each sample were individually aligned and clustered using Aligner and Complete Linkage Cluster tools of the RDP.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com