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Flexibility is linked to creativity and is important for functioning in a changeable world.
One can expect that the longer exons are more likely to retain their functionality after shuffling so they are important for function and maintained in evolution.
An increasing number of enzyme systems show the presence of conserved protein motions that are important for function.
Little genetic analysis has been done with any spanin so it is unclear which of these features are important for function.
If the predicted conserved regions are important for function, one would expect them to not be made of highly repetitive DNA, which occurs commonly in malaria intergenic regions.
By a systematic comparison of available sequences of homologs and their structures, they identify critical residues that are important for function.
Full activity requires GTP hydrolysis, suggesting that a conformational change of the factor is important for function.
Protonation of a buried water cluster has been shown to be important for functions of bacteriorhodopsin as well (Garczarek et al., 2005).
However, conformational variability is seen around Leu1706 which is a hot spot for cardiomyopathy mutations suggesting that this might be important for function.
While three-dimensional conformation may be important for function, it is not necessary for definition.
This conformational variability is likely to be important for function although there is no evidence that zinc is involved in vivo.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com