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If the Dfs are being screened for antigen expression or phenotype, they are immediately fixed and stained with the appropriate antibody.
Core biopsies are immediately fixed in formalin while surgical samples are often stored on ice for varying lengths of time before commencing formalin fixation.
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Cells were immediately fixed in a fixative solution as methanol acetic acid (6:1) 2 times.
Skin samples for immunostaining were immediately fixed in cold Zamboni's fixative and kept at 4°C overnight.
It said these cases had been "immediately fixed on discovery".
"It was a theoretical problem that was immediately fixed," Sebelius said.
The muscle biopsy contained within the clamp was immediately fixed in Formalin for subsequent sarcomere length measurement.
He said that eight were confirmed to have live current running through them and were immediately fixed.
Left lungs were detached from the mice and were immediately fixed with 4% paraformaldehyde before embedding with paraffin wax and routine processing.
The samples were immediately fixed in 4% paraformaldehyde for 25 min and labelled with specific antibodies as for immunochemistry described previously.
Samples were immediately fixed in 10%% neutral-buffered formalin.
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