The organs were homogenized by using a glass homogenizer.
mRCC were homogenized by using a Dounce homogenizer in a solution containing: 250 mM sucrose, 1 mM EDTA, 10 mM Tris-HCl, pH 7.6, 0.1 mg/mL PMSF, 100 mM β-mercaptoethanol, and Protease Inhibitor Cocktail (P8340, Sigma, USA).
Tissues were homogenized by using a Polytron PT3000 homogenizer (Kinematica).
Tissue pools were homogenized by using a Precellys bead mill homogenizer (Bertin Technologies, Ann Arbor, MI, USA), and DNA was extracted by using the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) according to manufacturer's instructions.
To separate and enrich ER membranes, Neuro2a cells were homogenized by using a stainless steel ball-bearing homogenizer in 0.25 M sucrose, 10 mM Tris-HCl, pH 7.4, 1 mM magnesium acetate, and a protease inhibitor mixture in a final concentration of 1 volume of cell pellet per 5 volumes of homogenizing medium.
Gastric biopsy specimens were homogenized by using a micropestle in a microcentrifuge tube, and the homogenates were added to HIA with 5% rabbit's blood and Skirrow media and incubated at 37°C microaerobically for up to 2 weeks.
The nanoparticles are de-agglomerated by using an ultrasonic disrupter after mixing with a base fluid and were homogenized by using magnetic force agitation.
Tissue samples were homogenized by using a glass homogenizing tube and with a Teflon-coated pestle.
After thawing and clearing from connective tissue, muscle was homogenized by using lysis buffer (2 mM Tris-HCl, 20 mM Hepes, 1 mM EDTA, 70 mM sucrose and 220 mM Mannitol) supplemented with 1 mM PMSF and protease inhibitor cocktail 1∶50 (Sigma) in a 1∶9 volume-to-weight ratio, followed by centrifugation for 10 min at 700×g to eliminate debris and nuclei.
Liver tissue (≈3 mg) was homogenized by using a bead mill.
