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Although not as well characterized, soluble cargo proteins including procathepsin Z and alkaline phosphatase contain export signals that are functional only when folded [28], [29].
In fact, many proteins are functional only when assembled into a protein complex [ 3- 5].
Most proteins are functional only when they are assembled into a protein complex and interact with other proteins in this complex.
Our results indeed show that desert EFNs are functional only when new vegetative and reproductive structures are developing, therefore increasing ant visitation at a time when these structures are most vulnerable to herbivore damage.
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Yet, in both species nitrogen fixation occurred (heterocytes were functional) only when nitrogen was limiting, and nitrogen fixation increased significantly at higher phosphorus concentration.
Traditional pulsating heat pipe with fewer turns is functional only when the heat source is placed at the bottom while the heat sink is on top.
Simultaneous viewing of a group of genes is essential to highlight in a robust way locational bias, which in this case has a very clear meaning: the TATA box is functional only when it appears near the TSS.
A secretory protein is functional only when it reaches the appropriate cellular compartment.
The process of cytoplasmic Ca2+/H+ exchange, which accumulates Ca2+ in acidic microdomains, is functional only when pHi is non-uniform.
The B class proteins in Arabidopsis and other species are reported to form heterodimers and to be functional only when both are present [ 41- 43].
TAP pulldowns involving Gpn3 TAP required growth in yeast media containing 2% galactose as the N-terminally tagged Gpn3 was functional only when overexpressed from a GAL1 promoter.
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