This BrdU injection would be expected to label mainly dividing cells but fewer DCX+, NeuN+ and Prox1+ cells, which would be mostly post-mitotic.
In this study, we employed a fmrf-GAL4 driver [62] to express GFP, and a polyclonal anti-RFamide serum [63] that is expected to label FMRFa-like peptide 2 7 and probably also other peptides containing RFamide in its C-terminus in Drosophila.
70 kDa dextran is expected to label predominantly macropinosomes due to size constraints of entry into endocytic structures.
The labeling method used here is expected to label functional presynaptic terminals that are capable of synaptic vesicle exocytosis and endocytosis.
This extended incubation is expected to label dividing NeuroD+ endocrine precursors and dividing duct cells whose progeny differentiated into NeuroD-expressing endocrine cells.
Since oligo-dT priming is expected to label mRNAs, including unspliced pre-mRNAs having poly(A) tails, whereas random priming is expected to label all RNAs in a cell, these results indicate that most of the transcripts originating from the AFAS regions (AFAS transcripts) are poly(A)-.
This antibody was generated against a SCGB 1A1 peptide, which is shared by both SCGB 1A1 and SCGB 1A1A, and therefore was expected to label both predicted SCGB 1A1 proteins.
Presynaptic marker VGlut1 is expected to label the majority of glutamatergic presynaptic terminals in the cortex [ 25], and we have previously shown its usefulness for immunostaining human presynaptic terminals in array tomography experiments [ 26].
To determine the presence and distribution of dendritic cells, sections of lymph node, spleen, and skin, where dendritic cells are expected to reside, were labeled by immunohistochemistry with MHC class II, the dendritic cell marker CD1a and the activated dendritic cell marker CD83.
The food and biotech industries are expected to fight the labeling initiative with a multi-million dollar statewide PR blitz, like the one which helped to defeat a similar measure in Oregon in 2002.
