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Long interspersed nuclear elements (LINEs) are non-LTR retrotransposons that are transcribed into mRNA by RNA polymerase II and encode two proteins that are essential for transposition [264]: a protein that binds to single-stranded RNA [265] and a protein with reverse transcriptase and endonuclease activity [266,267].
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BLAST analyses of the regions flanking the resolvase failed to reveal a repeat region between the two genes that would have been essential for transposition.
Both domains are thought to be essential for transposition.
Two of these transcripts, tnpA and tnpD, encode proteins that have been shown to be essential for transposition [ 8, 9].
They showed that the ZF motif, as well as the HTH motif, is essential for transposition promoted by IS1transposase because of their involvement in binding specifically to TIRs.
MURA showed high similarity to bacterial transposase and the virus integrase (Walbot and Rudenko 2002); hence, it is essential for transposition.
Terminal regions are known to be essential for transposition, so the internal region may evolve much more quickly than the terminal sequences.
The helitron helicase-like and Pif1-like helicase domains are present in the putative autonomous elements of every species and are under selective pressure because they are essential for helitron transposition.
Dinner reservations are essential for weekends.
Some items are essential for certain characters.
Good shoes are essential for working out.
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