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Kinetochores are therefore complex dynamic structures that are essential for multiple structural, functional and regulatory tasks during cell cycle progression.
Microtubules are polymers of α- and β-tubulin and are essential for multiple cellular activities, including meiotic and mitotic spindle function.
Microtubules and their dynamic behaviour are essential for multiple steps in mitosis and as such represent good antimitotic targets.
The protein kinase C inhibitor Ro 31-8220 (Ro) was included as a positive control, as ROS generation is known to be regulated by protein kinase C. Transient increases in intracellular calcium are essential for multiple neutrophil functions.
Mitochondria are essential for multiple processes within most cells, due to their role in the provision of energy and calcium homeostasis, as well as their role in apoptosis, either for the clearance of damaged cells or in programmed cell death.
Additionally, ROCK proteins are essential for multiple aspects of both the intrinsic and extrinsic apoptotic processes, including regulation of cytoskeletal-mediated cell contraction and membrane blebbing, nuclear membrane disintegration, modulation of Bcl-2 family member and caspase expression/activation and phagocytosis of fragmented apoptotic bodies [ 34].
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As we discussed in previous sections, apoptosis is essential for multiple physiological processes.
It is a small and highly basic protein, which is essential for multiple stages of the viral replicative cycle, with its structure preserved in all viral strains, including clinical isolates.
Any disruption of this interaction would prevent re-phosphorylation of PKC which is essential for multiple functions including fatty acid synthesis.
The hedgehog (Hh) signalling pathway is known to be essential for multiple aspects of embryonic development.
DOI: http://dx.doi.org/10.7554/eLife.02270.015 We report that Pak2, an effector for the small GTPase Rac and Cdc42, is essential for multiple stages of thymocyte development and maturation.
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