To obtain splenocytes and thymocytes, spleens were disaggregated using a cell strainer (Nunc), and resuspended in complete medium, comprising RPMI supplemented with 100 mM Hepes buffer pH 7.5 (BioWhitaker), 2 mM L-Glutamine (GIBCO), 1 mM sodium pyruvate (GIBCO), a commercial mix of non-essential amino acids (BioWhitaker), 0.1% β-mercaptoethanol (Sigma) and antibiotic/antimycotic (GIBCO).
Tissue was disaggregated using a mixture of type IV collagenase and DNase as previously described (Herbert et al., 2010).
Embryos were disaggregated using a pestle inside a 1.5 ml tube, gently resuspended and centrifuged for 10 minutes at 500 g.
The operation of individual loads is disaggregated using signal processing and presented as time-based load activity and statistical indicators.
The sale price of the bundled good irrigated farm property can be disaggregated using hedonic analyses to reveal the implicit price paid for the water component of the transaction.
For flow cytometry, one whole plate of d9 hEBhEB (whole well contents) were disaggregated using TrypLE and stained as above.
Subsequently, sections were disaggregated using pipettes, centrifuged, and resuspended in inhibitor solution (1 mg/ml ovomucoid, 1 mg/ml BSA, 0.0005% DNase I in Ca2+/Mg2+-containing EBSS, Worthington Biochemical Corporation).
For flow cytometry analysis, colospheres were disaggregated using trypsin.
Once dissected from the meninges, the tissue was disaggregated using trypsin and DNaseI.
Briefly, after removal of the meninges, brains of newborn Sprague-Dawley rats were disaggregated using nylon sieves (120 mm pore size) and seeded in 50-ml tissue culture flasks in (D MEM containing 20% heat-inactivated FCS.
