For 36 k whole genome mouse and human array performances both reference and test aRNA were directly labeled using ULS aRNA Fluorescent Labeling kit (Kreatech) with Cy3 for reference and Cy5 for test samples and co-hybridized to the slides [ 49]. 17 k human cDNA arrays were carried out as described according to our standard method for labeling and array hybridization [ 62].
For 36k human array hybridization, a two color system was used; both reference and test aRNA were directly labeled using ULS aRNA Fluorescent Labeling kit (Kreatech Diagnostics, Amsterdam, The Netherlands) with Cy3 for reference and Cy5 for test samples and co-hybridized to the slides.
Between smears, one group was directly labeled with Hoechst after air drying (DRIED), and the other were fixed either with glutaraldehyde (GLUT), or with methanol (MET), and labeled with Hoechst afterward.
Between smears, one group was directly labeled with Hoechst after air drying, and the other was fixed either with glutaraldehyde (GLUT), or with methanol, and afterward labeled with Hoechst.
(Intentionally satirical 'Twitter fakes' (aka parody accounts) should not be caught in this net, as Twitter has had a longstanding policy of requiring parody and fan accounts to be directly labeled as such in their Twitter bios).
I-125 was directly labeled to tyrosine of proteins; low-energy γ-ray can be easily detected by a radio detector but does not lead to self-radiolysis [9].
The antibodies and AnnexinV were directly labeled with FITC, PE, PerCP, PE-Cy7, Cy5, APC or APC-Cy7.
Five micrograms of sample RNA were directly labeled with either Hy3 or Hy5 using the miRCURY LNA array labeling kit (Exiqon).
Three hundred nanograms of small RNAs from tissue samples were directly labeled with N-code miRNA labeling kit (Invitrogen) per vendor's protocols.
