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Since saponins are difficult to purify, the complete structures of only a few are known.
The other enzymes are present in low amounts in yeast culture and are difficult to purify.
Some of these could be used in designing well-defined substitute antibodies for the study of target molecules which are difficult to purify, available in low quantities, are unstable or have poor immunogenity.
The possibility to reliably analyse minute amounts of intact viruses by mass spectrometry opens new perspectives in analytical and functional lipid studies on a wider range of viruses including pathogenic human ones, which are difficult to purify in large amounts.
Small peptides are poorly characterized in all organisms because they are difficult to purify for biochemical characterization.
Antibodies against transmembrane cell surface proteins that are difficult to purify in a native conformation and against epitopes that are not visible with the current display technologies might benefit from this platform.
Similar(43)
However, these complex proteins were difficult to purify and synthesize, which hindered their biosensing applications.
In addition, the integrase, which is known to be difficult to purify because of low solubility, was purified simply and without solubilizing agents using our system.
Because it is difficult to purify an intact recombinant Pit protein, we have been unable to assess the GEF activity of Pit in vitro.
Because 11β-HSD 1 is a membrane protein with a very hydrophobic character, it is difficult to purify it in an active state.
It is difficult to purify sufficient snake venom PA (SV-PA) for clinical applications due to the low SV-PA content in venom.
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