Consistent with a role for Rrd1 in activating PP4, rrd1Δ mutants largely rescue the fusion growth defect and are defective for full Maf1 dephosphorylation in ND and rapamycin treatment.
Taken together, strains with mutations in PP2A components enhance the fusion growth defect and retain rapid Maf1 dephosphorylation, whereas strains with PP4 mutations suppress the fusion growth defect and are defective for full Maf1 dephosphorylation displaying the utility of the Maf1 Rpc160 fusion for revealing involved factors.
Thus, while rsp5 mutant strains exhibit broad defects in the heat-induced endocytic response, Δart1 mutant cells are defective for the turnover of only a subset of PM proteins.
RDE-4 facilitates processing of dsRNA by DCR-1, and rde-4 mutant animals are defective for RNAi [2], [13], [14].
ES cells lacking the Casp3 gene are defective for differentiation, which can be rescued by expression of a cleavage-resistant Nanog variant.
The noncytotoxic phenotype observed in six EZ derivatives indicates that these mutants are defective for translocation of ExoU into host cells.
The degradation of CFTR through ERAD in yeast is compromised in strains that are defective for Hsp70 function [67], yet CFTR was degraded in all our strains, although with different efficiencies (Figure 11).
ICP0-deficient mutants are defective for growth at low multiplicities of infection in cell culture, and are attenuated and fail to efficiently reactivate from latent infection in cell culture and in animals [32].
All tumor cell lines that have been tested are defective for Myc auto-repression, and have high levels of Myc produced from wild type loci and re-arranged loci.
ackA mutants are defective for the degradation of acetyl-P, pta mutants are defective its synthesis, pta ackA mutants are defective for both, and acs mutants are defective for the assimilation of small amounts of acetate (1).
The gi mutants are defective for the expression of CCA1 and LHY genes.
