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Total RNA was then quality controlled for integrity on a Bioanalyzer (Agilent Technologies).
The mechanism for maintaining telomere integrity is controlled by telomerase, a ribonucleoprotein enzyme that specifically restores telomere sequences, lost during replication by means of an intrinsic RNA component as a template for polymerization.
The analyses in this report were not controlled for treatment integrity, 32 33 that is, classes with low-treatment fidelity that have only been exposed to parts of the intervention contribute to the same extent to the results of the intervention group as classes with complete programme implementation.
RNA integrity was controled on Bioanalyzer (Agilent Technologies).
Unlike previous observations in sensory cortical areas (Pizzorusso et al. 2002), intracortical ChABC delivery after SCI perturbs anatomical and functional CST reorganization, suggesting that spatial changes in cortical PNN integrity must be tightly controlled for meaningful reorganization of cortical motor output.
β- actin was amplified to control for RNA integrity.
A common cellular gene (β-actin) was used as a control for integrity of RNA template.
Phase contrast images were acquired prior and after each experiment to control for cellular integrity.
The probes were tested for integrity by running negative controls of just water and probe at low and high probe concentrations.
To control for the integrity of the DNA, the cellular beta-globin gene was amplified.
TEER and CCL values before and after the assays were monitored continuously for integrity control.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com