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Controls are continuously selected as a 2% representative sample of all live births in Barcelona hospitals, by random date of birth sampling, independent of the cases (nonmatched).
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The parasite cultures were continuously selected for binding to CSA by panning on BeWo cells, resulting in surface expression of VAR2CSA and sex specific recognition by IgG from human female serum, compared to male and Danish serum (data not shown).
The transduced cells were continuously selected in G418-containing media.
During the study period, samples were continuously selected for genotyping.
The individual clones on the selection medium could be obtained after 25 to 30 days and they were continuously selected once per month for at least eight times.
Infected cells (SNCA_Tri-C1_KD and SNCA_Tri-C2_KD) were continuously selected with puromycin and showed lower levels of aSyn compared with non-targeting shRNA control (SNCA_Tri-C1_Scr) cells, which demonstrated unaltered aSyn levels.
The plant material utilized for nucleosome distribution analysis resulted from multiple generations of backcrossing to introgress the mop1 1 mutation into the B73 reference genome, but as mop1 1 and other associated genetic loci related to plant pigmentation were continuously selected for, a certain amount of genetic variability persisted in segregating populations.
This exponential growth phase probably occurs after the formation of the parental clone, as subclones are continuously created and selected for and as they adapt to the dynamic microenvironment.
Although only plate #1 could be imaged continuously, selected plates from other positions were photographed at the end of the experiment as part of the astronaut harvest operations on orbit.
Cells were subsequently continuously selected in G418-containing media to enrich for successfully transduced cells.
"They are continuously learning new methods".
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