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Our results showed that the decreased mTOR/AKT signaling pathway and increased AMPK signaling pathway were continuously maintained by 2DG until the last time points of sample preparation (Fig.  3b).

The chimeric structure of EMR2 has been continuously maintained since early mammalian radiation by gene conversion events between different regions of the EMR2 gene and the oppositely orientated and physically adjacent genes CD97 and EMR3 [ 15].

Anesthesia was continuously maintained with isoflurane and body temperature was maintained at 37°C by an immersion thermostat throughout the experiment.

The irrigated moisture was continuously maintained at field capacity.

From this time until 1923, a garrison was continuously maintained at the castle.

A level of salivation is continuously maintained in cockroaches [24].

Oli-neu cells were continuously maintained at 37°C in 5% CO2 at a humid atmosphere.

Thereafter, hNSC and IhNSC were continuously maintained in the respective oxygen concentrations.

It is continuously maintained until adult stage.

It has been continuously maintained for more than 20 years.

In the third experimental schedule, insects were continuously maintained under long-day conditions.