The cell lysate, which could only be concentrated up to a concentration of 80 mg mL−1, shows no significant compression effect at its highest concentration.
Our structural studies were initiated with proteins that contained N-terminal His tags, and surprisingly, we found that the His-tagged P23T mutant protein could be concentrated up to 90 mg/mL (no further concentration was attempted).
In the first MD step seawater is concentrated up to the saturation point of CaCO3.
Furthermore CO2 in the exhausted gas is required to be concentrated up to more than ca. 30%.
With low pH of the raw water (pH 2.2) in the first stage, chromate was presented as HCrO4− and monovalent ions (HCrO4−, NH2SO3−, Na+ and Cl−) were able to pass through the membrane thus chromate was concentrated up to 191%.
To concentrate and exchange the buffer, purified CAHL-C was concentrated up to 40 times with PBS by using Amicon Ultra 30 (Millipore, EMD, Germany).
The collected media were concentrated up to 500 X (8.0 ug/ul), using a cut-off filter of 3 kDa (Vivian-science).
Fractions containing the antibody-receptor complex in equimolar stoichiometry were combined, and the protein solution was concentrated up to 16.4 mg ml−1 in 10 mM HEPES pH 7.4, 150 mM NaCl using ultrafiltration.
The mixture was concentrated up to one third of its total volume.
48 h-old algal cultures were concentrated up to a 500 μg.mL−1 dry biomass to optimize resolution of functional measurements.
