To summarize these results, we found that the directed mutation of the trypsinogen surface charge led to an almost complete loss of trypsinogen autoactivation, while the enzyme could still be completely activated by its natural activation enzyme human enteropeptidase.
The Hall effect measurements showed that the hole concentration (1.942 × 1019 cm−3) is almost twice the average Cr concentration (1 × 1019 confirmingfirming that almost all of the Cr atoms were completely activated to the double acceptor state by the laser-doping process without requiring any additional annealing step.
At 'threshold +10 nA' stimulations, the AIS component was completely activated during the stimulation pulse.
Consisted with this possibility, we found that CD4 peripheral T cells from the Foxn1Δ/Δ mice could not be completely activated.
Also, PMA might not be completely activated due to the unknown light spectrum that halogen lamp emitted.
After 48 h caspase-9 was completely activated (3-fold at IC50 and 4-fold at IC80).
In vitro, DNR-GA3 was 18 times less toxic than daunorubicin (DNR) and the prodrug was completely activated to the parent drug by human beta-glucuronidase.
This novel prodrug was completely activated to the parent drug by human β-glucuronidase with V max= 25.0 μmol min 1mg 1and K m= 1100 μM.
Previously reported conditions for generating trypsin-α2M vary greatly [2,5,20]; therefore, in this study we used an optimized method to obtain preparations of trypsin-α2M that were completely activated but not degraded (Supplementary Fig. 1).
The biochemical and physiological data showed that the plants incubated for the last 30 h in the presence of 10 mM NO3- were in a condition in which nitrogen metabolism is completely activated in both root and leaf organs and that, at the same time, no stress symptoms were detectable in the control plants.
Additionally, we find that cascades operating under zero-order ultrasensitivity [17], in which the cascaded signal is either completely activated or not active at all, can serve to slow down signal propagation in a cell, even as the signal is amplified overall.
