A fusion protein containing GFP and the N-terminal region of the second chloroplast-type RPL10 copy from Arabidopsis was clearly localized to mitochondria (Fig. 4B D, and yellow arrowhead), suggesting its mitochondrial localization in vivo.
The GFP protein alone accumulated in the cells without specific localization; however, GFP from the GFP-tagged Sl3-MMP protein was clearly localized on the plasma membrane before and after plasmolysis and no GFP was detected in the space between plasma membrane and cell wall (Fig. 11).
This is most apparent when examining hZW10 localization upon treatment with the MT depolymerizing compound vinblastine, where hZW10 is clearly localized to kinetochores but completely absent from spindle poles.
For the HER observatory, the phase transition marked T2 is clearly localized by the scaling exponent, displaying a persistent behaviour reaching a maximum value of β(t) ≈ 3 at 19 00 UT.
In other cases, CSF-derived DCs were clearly localized within parenchymal inflammatory foci.
RFP-RhoB was clearly localized inside Lamp1-GFP positive vesicles and GFP-RhoB-positive vesicles showed LTR staining (Fig. 2c).
Thus, the TMS effect can be clearly localized on the scalp and appears to affect all three points in space.
In Pro-MI and latePro-MI stages, overexpressed Bub3 was clearly localized at the kinetochores of chromosomes, which was the same as the endogenous Bub3 in Fig. 1Bc, d.
Immunofluorescence assay with the anti-LdUfc1 antibodies showed that endogenous Leishmania Ufc1 protein in promastigotes is clearly localized in mitochondria (Figure 5E, bottom panel).
