This suggests that the AHLs used in this study were capable of insertion into the plasma membrane of Jurkat T-lymphocytes leading to changes in the dipole potential.
Interestingly, pSLE1 contains two genes encoding putative integrases, suggesting that the plasmid might be capable of insertion into the chromosome.
To conclude, we show that each hydrophobic domain of TPCs is capable of insertion and forming oligomers, probably reflective of the ancestral one-repeat channel from which it evolved.
In the present paper, we report that both domain I and II of human TPCs are capable of independent insertion into membranes, whereas the loop linking the domains fails to insert.
Based on the well characterized responses of the fluorescent probe we utilize in this study [30], [31] we are able to conclude from our observations that AHLs are capable of direct insertion into biological membranes in the micromolar concentration range.
Transposable elements (TEs) are DNA sequences that are capable of autonomous replication and insertion at new locations in a genome (Kidwell and Lisch 2001).
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De novo TE remobilization and insertion are capable of significantly influencing genome stability, host gene transcription, splicing or RNA editing.
While short insertions are easy to reconstruct (as seen in Section 1.2), to the best of our knowledge, only a few methods are capable of handling medium or long insertions.
These L1 insertions are capable of altering the genome in myriad ways by disrupting genes, altering splicing, increasing the frequency of recombination, and negatively affecting the stability and integrity of the genome because of their ability to create breaks in genomic DNA during the process of mobilization or retrotransposition [ 13– 15].
