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Here, we demonstrate a method to engineer highly selective synthetic antibodies (sABs) against LD2 and LD4 that are binding sites for focal adhesion kinase (FAK) and other proteins.
Although a set of four hexanucleotide (H) sequences to the right of the palindrome (organized in two tandem repeats: the proximal H1/H2 and the distal H3/H4) are binding sites for the viral Rep-associated proteins in vitro, only a proximal tandem (H/H or h-like/H) is essential for PCV1 DNA replication.
Post-transcriptional regulation requires a signal on the target mRNA, usually in the form of sequence or structural motifs present in the UTRs that are binding sites for a trans-acting factor.
For the case of a transcriptional activator, there are binding sites for the transcription factors upstream of the regulated gene, and when these sites are occupied transcription proceeds at some rate, but when the site is empty transcription is inhibited.
HSEs are binding sites for HSFs (heat-shock factors, Figure 1), transcription factors whose trimerization mediates the heat-shock response in eukaryotes [11], [12] in combination with numerous co-regulators [13].
To evaluate the changes that occur to the transcription site chromatin during activation, we immunostained inactive and transcriptionally activated cells using antibodies against histone H4 acetyl-K5, histone H4 acetyl-K12, and histone H3 acetyl-K9, which are binding sites for the acetyl-lysine binding, Brd2 and Brd4 proteins [10], [17], [44], [45], [46].
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This 2 kb region contains 237 bases covered by potential TFBS, many of which were binding sites for Sp1.
The purpose of evaluating the regions of disorder within a protein is that such areas are observed to be binding sites for protein-ligand interactions.
Such regions of disorder within proteins are observed to be binding sites for proteins assuming a secondary structure that is observed under x-ray crystallography when in association with the partner ligand [23], [24].
Basically, they were binding sites for general, commonly found, transcription factors.
Lys158 and Phe300 may also be binding sites for Asc as described below.
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